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Immunogen
A synthesized peptide derived from human SOX2
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Antibody s target
Human SOX2
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Antibody s full description
Rabbit Anti-Human SOX2 Polyclonal
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Primary research fields
Stem Cells, Embryonic Stem Cells, ESC Intracellular Markers, Stem Cells, Germline Stem Cells
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
SOX 2 Antibody, SRY (sex determining region Y) box 2 Antibody, SRY related HMG box 2 Antibody, SRY-box 2 Antibody, ANOP3 Antibody, ysb Antibody
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Verified applications
WB, IHC, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~34kDa, endogenous levels of total SOX2
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1287 was sufficient for detection of SOX2 in 10 µg of HT29 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development when it forms complexes with Oct4 and Nanog. Varying levels of Sox2 can affect the determination of cell fate. Sox2 inhibits differentiation into the mesendoderm germ layer and promotes differentiation into neural ectoderm germ layer.
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Bibliography
1. "SOX2". NCBI. 2. Johansson H, Simonsson S (2010) Aging. (11): 815–822. 3. Thomson M., Liu S.J., Zou L.N., Smith Z., Meissner A., Ramanathan S. (2011) Cell. 145(6): 875–889.
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Released date
6/Sep/2016
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NCBI number
NP_003097.1
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Gene number
6657
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Protein number
P48431
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
SOX2, SOX2-OT
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Short name
Rabbit Anti- SOX2 Polyclonal: ATTO 680
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
ATTO 680
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman SRY (sex determining region Y)-box 2 polyclonal: ATTO 680
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
SRY (sex determining region Y)-box 2, ANOP3 and MCOPS3, SOX2 and IDBG-66692 and ENSG00000181449 and 6657, protein heterodimerization activity, nuclei, Sox2 and IDBG-137912 and ENSMUSG00000074637 and 20674, SOX2 and IDBG-647279 and ENSBTAG00000011598 and 784383
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