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Immunogen
Synthetic peptide of human c-KIT (AA119-133)
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Antibody s target
Human c-Kit
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Antibody s full description
Rabbit Anti-Human c-Kit Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
C Kit Antibody, CD117 Antibody, Kit Antibody, KIT oncogene Antibody, Mast cell growth factor receptor Antibody, p145 c-kit Antibody, PBT Antibody, Proto oncogene tyrosine protein kinase Kit Antibody, SCF Receptor Antibody, soluble KIT variant 1 Antibody, Steel Factor Receptor Antibody, tyrosine protein kinase Kit Antibody, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 109.865 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1155 was sufficient for detection of c-Kit in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane
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Tissue specificity
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells
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Scientific context
c-KIT is a receptor tyrosine kinase that plays an essential role in the regulation of cell survival and proliferation, especially hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and melanogenesis. Following activation by KITLG/SCF binding, it can activate the PLCG1, AKT1, RAS, and STAT pathways. Activation of PLCG1 can lead to increased calcium signalling. Mutations leading to constitutive kinase activity have been associated with gastrointestinal stromal tumour, testicular tumours, and acute myelogenous leukemia. The human proto-oncogene c-kit was first identified as the homolog of the feline sarcoma viral oncogene v-kit.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_000213
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Gene number
3815
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Protein number
P10721
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Short name
Rabbit Anti- c-Kit Polyclonal: ATTO 680
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 680
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman c-reagent polyclonal: ATTO 680
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Alternative technique
polyclonals, rabbit-anti, antibodies, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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