Rabbit Anti-Human RAF1 Polyclonal: ATTO 633
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Catalog numberSPC-1194D-A633
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Price:
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Size100 µl
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ImmunogenSynthetic peptide of human RAF1 (AA121-135)
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Antibody s targetHuman RAF1
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Antibody s full descriptionRabbit Anti-Human RAF1 Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other namec Raf Antibody, C-raf Antibody, C-Raf proto-oncogene, serine/threonine kinase Antibody, CMD1NN Antibody, Craf 1 transforming gene Antibody, cRaf Antibody, Craf1 transforming gene Antibody, EC 2.7.11.1 Antibody, kinase Raf1 Antibody, Murine sarcoma 3611 oncogene 1 Antibody, NS5 Antibody, Oncogene MIL Antibody, Oncogene RAF1 Antibody, OTTHUMP00000160218 Antibody, OTTHUMP00000207813 Antibody, OTTHUMP00000209389 Antibody, Protein kinase raf 1 Antibody, Proto-oncogene c-RAF Antibody, Raf 1 Antibody, Raf 1 proto oncogene serine/threonine kinase Antibody, RAF Antibody, Raf proto oncogene serine/threonine protein kinase Antibody, RAF proto-oncogene serine/threonine-protein kinase Antibody, RAF-1 Antibody, RAF1 Antibody, RAF1_HUMAN Antibody, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 Antibody, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV Antibody, v raf 1 murine leukemia viral oncogene homolog 1 Antibody, v-raf murine sarcoma viral oncogene homolog 1 Antibody, vraf1 murine leukemia viral oncogene homolog 1 Antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects ~73kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-1194 was sufficient for detection of RAF1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm, Cell Membrane, Mitochondrion, Nucleus
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Tissue specificityIn skeletal muscle, isoform 1 is more abundant than isoform 2
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Scientific contextRAF1 is a protein-serine/threonine kinase. RAF-1 is upregulated in many cancers since Ras (an upstream activator of RAF) is mutated/upreguated in many cancers. For example, RAF-1 is over-expressed due to oncogenic ras mutations in about 35% of Non-Small Cell Lung Cancer (NSCLC). A small number of RAF mutations have been found in stomach and mouth tumours. RAF-1 gene amplification was detected in 4% of bladder cancer samples, while deletions at the RAF-1 locus were detected in 2.2% of these samples; these amplifications and deletions were correlated with tumour grade.
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_002871
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Gene number5849
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Protein numberP04049
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolRNASE3, RAF1
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Short nameRabbit Anti- RAF1 Polyclonal: ATTO 633
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 633
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman v-raf-1 murine leukemia viral oncogene homolog 1 polyclonal: ATTO 633
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Alternative to gene targetv-raf-1 murine leukemia viral oncogene homolog 1, c-Raf and CMD1NN and CRAF and NS5 and Raf-1, RAF1 and IDBG-19277 and ENSG00000132155 and 102724915,5894, transferase activity, nuclei, Raf1 and IDBG-179428 and ENSMUSG00000000441 and 110157, RAF1 and IDBG-646381 and ENSBTAG00000004514 and 521196
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Gene info
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Identity
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Gene
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Long gene nameribonuclease A family member 3
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Synonyms gene
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Synonyms gene name
- ribonuclease, RNase A family, 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-02-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ribonuclease A family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRaf-1 proto-oncogene, serine/threonine kinase
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Synonyms gene name
- v-raf-1 murine leukemia viral oncogene homolog 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitogen-activated protein kinase kinase kinases
- RAF family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data