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Immunogen
Synthesized peptide derived from human HDAC1.
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Antibody s target
Human HDAC1
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Antibody s full description
Rabbit Anti-Human HDAC1 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
DKFZp686H12203 antibody, GON 10 antibody, HD1 antibody, HDAC 1 antibody, HDAC1 antibody, HDAC1_HUMAN antibody, Histone deacetylase 1 antibody, Reduced potassium dependency yeast homolog like 1 antibody, RPD3 antibody, RPD3L1 antibody
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Verified applications
WB, IHC, ICC/IF, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total HDAC1 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1324 was sufficient for detection of HDAC1 in 10 µg of NIH/3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Histone deacetylase 1 is an enzyme that in humans is encoded by the HDAC1 gene. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
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Bibliography
1. Taunton J. et al. (1996) Science 272 (5260): 408–11. 2. Gerdin AK. (2010) Acta Ophthalmologica 88 (S248).
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Released date
6/Sep/2016
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NCBI number
NP_004955.2
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Gene number
3065
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Protein number
Q13547
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324). Tissue: Lung Carcinoma Tissue. Species: Human. Primary Antibody: Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324). Tissue: NIH/3T3. Species: Mouse. Primary Antibody: Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~62kDa HDAC1 protein using Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-HDAC1 Polyclonal Antibody (SPC-1324) at 1:1000. Predicted/Observed Size: ~62kDa. Rabbit Anti-HDAC1 Antibody used in Immunohistochemistry (IHC) on Lung Carcinoma Tissue (SPC-1324) | Rabbit Anti-HDAC1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on NIH/3T3 (SPC-1324) | Rabbit Anti-HDAC1 Antibody used in Western blot (WB) on NIH/3T3 cell lysates (SPC-1324)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
HDAC1
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Short name
Rabbit Anti- HDAC1 Polyclonal: ATTO 700
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
ATTO 700
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman histone deacetylase 1 polyclonal: ATTO 700
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
histone deacetylase 1, this GO N-10 and HD1 and RPD3 and RPD3L1, HDAC1 and IDBG-95712 and ENSG00000116478 and 3065, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, HDAC1 and IDBG-644586 and ENSBTAG00000012698 and 404126
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