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Immunogen
Peptide derived from human p62 protein UBA domain (a.a 387-436)
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Antibody s target
Human p62
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Antibody s full description
Rabbit Anti-Human p62 Polyclonal
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Primary research fields
Cancer, Autophagy
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
SQSTM1 Antibody, EBIAP Antibody, p60 Antibody, Sequestosome-1 Antibody, Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody, Ubiquitin binding protein p62 antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:5000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Salt precipitated
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Recommended buffer for storage
PBS, 0.1% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Ubiquitin binding autophagy receptor protein.
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Storage recommendations
4°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:500 dilution of SPC-219 was sufficient for detection of p62 in human spleen tissue lysate by electrochemiluminescence analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cytoplasmic Vesicle, Autophagosome, Endosome, Late Endosome, Nucleus, Endoplasmic Reticulum
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
p62 is a scaffolding adaptor protein which is expressed under conditions of cellular stress. This protein has a variety of protein binding domains, allowing it to regulate a variety of signal transduction pathways. It also has an influence on protein trafficking, aggregation and degradation. p62 plays an important role in mediating autophagy.
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Bibliography
1. Wang L., Cano M., & Handa J. (2014) Biochim Biophys Acta. 1843(7): 1248-58.
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Released date
12/Jun/2014
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NCBI number
NP_714941.9
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Gene number
8886
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Protein number
Q13509
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
