quasa Real-time PCR Genotyping kit for BRAF (V600E) (50 reactions)[BRAF (V600E) (50 reactions)]

• Catalog number
  MBS486016
• Price
  Please ask
• Size
  NA

• Products_type
  PCR Kit
• Group
  PCR, polymerase chain reaction
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
• Description
  Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.

• Gene target
  quasa   Genotyping   kit   for   BRAF   V600E   [BRAF   V600E  
• Gene symbol
  BRAF
• Short name
  quasa Real-time PCR Genotyping kit for BRAF (V600E) (50 )[BRAF (V600E) (50 )]
• Technique
  Real-time, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
• Alternative name
  quasa Real-time PCR test kit Genotyping reagent to measure v-raf murine sarcoma viral oncogene homolog B1 (V600E) (50 reactions)[v-raf murine sarcoma viral oncogene homolog B1 (V600E) (50 reactions)]
• Alternative technique
  rtpcr, kits, dna-amplification
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:1097
• Gene
  BRAF
• Long gene name
  B-Raf proto-oncogene, serine/threonine kinase
• Synonyms gene name
  • v-raf murine sarcoma viral oncogene homolog B
• Synonyms
  • BRAF1
• GenBank acession
  • M95712
• Locus
  7q34
• Discovery year
  1991-07-16
• Entrez gene record
  673
• Pubmed identfication
  • 2284096
  • 1565476
• RefSeq identity
  • NM_004333
• Classification
  • Mitogen-activated protein kinase kinase kinases
  • RAF family
• VEGA ID
  OTTHUMG00000157457
• Locus Specific Databases
  • LRG_299

MeSH Data

• Name
  Real-Time Polymerase Chain Reaction
• Concept
  Scope note: Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
• Tree numbers
  • E05.393.620.500.706
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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