quasa Real-time PCR Genotyping kit for JAK2(V617F) (50 reactions)
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Other size
please contact us to order other different size
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Description
Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.
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Gene target
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Gene symbol
JAK2
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Short name
quasa Real-time PCR Genotyping kit for JAK2(V617F) (50 )
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Technique
Real-time, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Alternative name
quasa Real-time PCR test kit Genotyping reagent to measure Janus kinase 2(V617F) (50 reactions)
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Alternative technique
rtpcr, kits, dna-amplification
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
Janus kinase 2
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Synonyms
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Locus
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Discovery year
1992-04-16
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Entrez gene record
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Pubmed identfication
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Classification
- FERM domain containing
- Jak family tyrosine kinases
- SH2 domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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