Platinum-E Retroviral Packaging Cell Line, Ecotropic
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Catalog number
RV-101
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Price
Please ask
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Size
1 vial
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We send this reagant on
Dry Ice
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The temperatureerature for storage is
For best results begin culture upon receipt. If this is not possible, store at -80C until first culture. Store subsequent cultured cells in liquid nitrogen.
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SDS or safty data sheet
more (RV-101, 102, 103) GHS.pdf
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Type of reagent
Viral Expression
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Related techniques
Viral Expression, Retroviral Expression, Retroviral Expression and Packaging, Platinum Retroviral Packaging Cell Lines
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Use of the reagent
Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.
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Cellular use
Higher retroviral yields: average titer 106 to 107 infectious units/mL with transient transfection
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Protocol information
Longer stability: up to 4 months in the presence of drug selection
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Reagent properties
Produces ecotropic retrovirus, which can only readily infect mouse or rat cells
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Living modified organisme in this reagent or LMO
Human cells transduced with retroviral genes
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Conjugation and form
host specific
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Species compatible to
human cells and cell lines and other cells
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Use of this test kit or product
Molecular biology
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Antigen
the sequence of the protein
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,39
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Molecular weight
kiloDalton
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This reagent is purified
0.98
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Description
For cells, cell lines and tissues in culture till half confluency.
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Gene target
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Short name
Platinum-E Retroviral Packaging Line, Ecotropic
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Alternative name
Platinum-E Retroviral Packaging cellular Line, Ecotropic
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Tissue
cell
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MeSH Data
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Name
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Concept
Scope note:
Process of growing viruses in live animals, plants, or cultured cells.
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Tree numbers
- E01.370.225.875.970
- E05.200.875.970
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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