Platinum-A Retroviral Packaging Cell Line, Amphotropic

Shipping Temp

Dry Ice
Storage Temp

For best results begin culture upon receipt. If this is not possible, store at -80C until first culture. Store subsequent cultured cells in liquid nitrogen.
Category Product Type

Cell Line
Technical data
Data sheet

more (RV-101, 102, 103) GHS.pdf
UNSPSC

41106514
Product Line

Viral Expression
Short Description

Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.
Product feature 1

Higher retroviral yields: average titer 106 to 107 infectious units/mL with transient transfection
Product feature 2

Longer stability: up to 4 months in the presence of drug selection
Product feature 3

Produces amphotropic retrovirus, which can infect human, mouse and rat cells
LMO Content

Human cells transduced with retroviral genes
Description

For cells, cell lines and tissues in culture till half confluency.

Name

Virus Cultivation
Concept

Scope note: Process of growing viruses in live animals, plants, or cultured cells.
Tree numbers
- E01.370.225.875.970
- E05.200.875.970
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data