Anti-DsbA Antibody (1A2-D4-G10)

• Catalog number
  A1326-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Thiol:disulfide interchange protein DsbA
• Target Alternative Name
  Thiol:disulfide interchange protein DsbA, dsbA, dsf, ppfA
• Antibody Type
  Monoclonal
• Clone
  1A2-D4-G10
• Species Reactivities
  E.coli
• Immunogen Sequence
  Recombinant E.coli. DsbA protein fragments over expressed in E.coli
• Accession
  P0AEG4
• Gene Id
  8114936
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
• Purification
  Affinity purified
• Application
  WB
• Positive Control
  WB: AKT1, AKT2, AKT3 and DSBA recombinant antigen
• Application And Usages
  WB; 1:1000
• Description
  Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). mgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  DsbA   1A2-D4-G10  
• Gene symbol
  ZBTB12, DPF2, DPF1, DPF3, BUD31
• Short name
  Anti-DsbA Antibody (1A2-D4-G10)
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG2b
• Alternative name
  Antibody toDsbA (antibody to-) (1A2-D4-G10)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:19066
• Gene
  ZBTB12
• Long gene name
  zinc finger and BTB domain containing 12
• Synonyms gene
  • C6orf46
• Synonyms gene name
  • chromosome 6 open reading frame 46
• Synonyms
  • G10
  • NG35
  • D6S59E
• GenBank acession
  • BC043609
• Locus
  6p21.33
• Discovery year
  2002-08-13
• Entrez gene record
  221527
• Pubmed identfication
  • 9545376
• RefSeq identity
  • NM_181842
• Classification
  • BTB domain containing
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000031169

Gene info

• Identity
  HGNC:9964
• Gene
  DPF2
• Long gene name
  double PHD fingers 2
• Synonyms gene
  • REQ
• Synonyms gene name
  • requiem, apoptosis response zinc finger gene
  • D4, zinc and double PHD fingers family 2
• Synonyms
  • ubi-d4
  • BAF45d
  • SMARCG2
• GenBank acession
  • U94585
• Locus
  11q13.1
• Discovery year
  1997-05-15
• Entrez gene record
  5977
• Pubmed identfication
  • 11845289
• RefSeq identity
  • NM_006268
• Classification
  • BAF complex
  • PHD finger proteins
• VEGA ID
  OTTHUMG00000165985

Gene info

• Identity
  HGNC:20225
• Gene
  DPF1
• Long gene name
  double PHD fingers 1
• Synonyms gene name
  • D4, zinc and double PHD fingers family 1
• Synonyms
  • neuro-d4
  • NEUD4
  • BAF45b
  • SMARCG1
• GenBank acession
  • U43843
• Locus
  19q13.2
• Discovery year
  2003-01-28
• Entrez gene record
  8193
• Pubmed identfication
  • 8812431
• Classification
  • PHD finger proteins
  • BAF complex
• VEGA ID
  OTTHUMG00000157164

Gene info

• Identity
  HGNC:17427
• Gene
  DPF3
• Long gene name
  double PHD fingers 3
• Synonyms gene name
  • D4, zinc and double PHD fingers, family 3
• Synonyms
  • cer-d4
  • Cerd4
  • FLJ14079
  • BAF45c
  • SMARCG3
• GenBank acession
  • U43919
• Locus
  14q24.2
• Discovery year
  2003-01-28
• Entrez gene record
  8110
• Pubmed identfication
  • 11845289
  • 8812431
• Classification
  • PHD finger proteins
  • BAF complex
• VEGA ID
  OTTHUMG00000171364

Gene info

• Identity
  HGNC:29629
• Gene
  BUD31
• Long gene name
  BUD31 homolog
• Synonyms gene
  • G10
• Synonyms gene name
  • G10 maternal transcript homolog (Xenopus laevis)
  • BUD31 homolog (yeast)
  • BUD31 homolog (S. cerevisiae)
• Synonyms
  • YCR063W
  • EDG-2
  • EDG2
  • fSAP17
  • Cwc14
• Synonyms name
  • functional spliceosome-associated protein 17
• GenBank acession
  • BC022821
• Locus
  7q22.1
• Discovery year
  2006-03-10
• Entrez gene record
  8896
• Pubmed identfication
  • 7841202
• RefSeq identity
  • NM_003910
• Classification
  • Spliceosomal C complex
  • NTC associated proteins
  • Spliceosomal B complex
  • Spliceosomal Bact complex
  • Spliceosomal P complex
• VEGA ID
  OTTHUMG00000154602

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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