Mouse Anti-Mouse Thorase/Atad1 Monoclonal IgG1, Clone S125-10
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Catalog numberSMC-347D-A633
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PricePlease ask
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Size100 µg
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CloneS125-10
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ImmunogenFusion protein amino acids 1-361 (Full length) of mouse Thorase
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Antibody s full descriptionMouse Anti-Mouse ATAD1 ATPase Monoclonal IgG1 Antibody, Clone: S125-10: ATTO 633
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameATAD1 antibody, AAA+ ATPase Thorase antibody, Ab2-088 antibody, AFDC1 antibody, ATAD1_HUMAN antibody, ATPase family AAA domain containing protein 1 antibody, ATPase family AAA domain-containing protein 1 antibody, ATPase family, AAA domain containing 1 antibody, ATPase Thorase antibody, AW107648 antibody, FLJ14600 antibody, FNP001 antibody, Thorase antibody
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Raised inMouse
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Antibody s targetATAD1 ATPase
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Primary research fieldsCancer, Neuroscience, Glutamate Receptors, AMPA Receptors
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~40kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-347 was sufficient for detection of Thorase in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellPeroxisome
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThorase, also known as Atad1 or ATPase family AAA domain-containing protein 1 is involved in synaptic plasticity, learning and memory through the regulation of the surface expression of AMPA receptors (AMPAR). Thorase is widely expressed with the highest expression in the brain and testis.
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Bibliography1. Genes to Systems Breast Cancer Database (G2SBC): www.itb.cnr.it/breastcancer/php/geneReport.php?id=84896
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Released date13-Apr-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_080763.2
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Gene number67979
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Protein numberQ9D5TO
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10 (SMC-347). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody (SMC-347) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and accumulates around nucleus. Western Blot analysis of Rat brain membrane lysate showing detection of ATAD1 ATPase protein using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10 (SMC-347). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody (SMC-347) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10 (SMC-347). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody (SMC-347) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining in all basal cells. Mouse Anti-ATAD1 ATPase Antibody [S125-10] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-347) Mouse Anti-ATAD1 ATPase Antibody [S125-10] used in Western Blot (WB) on Rat brain membrane lysate (SMC-347) Mouse Anti-ATAD1 ATPase Antibody [S125-10] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-347)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD115-10, PIRC49, SNORD116-10, IGKV2OR2-10, IGHV3OR16-10, ERVK-10
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Short nameMouse Anti-Mouse Thorase/Atad1 Monoclonal IgG1, Clone S125-10
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 633
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Thorase/Atad1 monoclonal IgG1, clonality S125-10
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Alternative techniquemurine, antibodies
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Clone nameS125-10
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-10
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 49
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-10
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2/OR2-10 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2/OR2-10
- immunoglobulin kappa variable 2/OR2-10 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa (IGK) orphons
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-10 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR16-10
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 10
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Synonyms gene name
- endogenous retrovirus group K, member 10
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Synonyms name
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GenBank acession
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Locus
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Discovery year2011-05-05
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Entrez gene record
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Pubmed identfication
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
