MOUSE Anti-HUMAN CD169:FITC Antibody
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Catalog numberGEN216211
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Price:
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Size0.025 miligrams
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Also known asCD169
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Other namessialoadhesin; Sialoadhesin; sialoadhesin; sialic acid-binding Ig-like lectin 1; sialic acid-binding immunoglobulin-like lectin 1; sialic acid binding Ig-like lectin 1, sialoadhesin; Sialic acid-binding Ig-like lectin 1
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameCD169
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Gene name synonimsN/A
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Other gene namesSIGLEC1; SIGLEC1; SN; CD169; SIGLEC-1; dJ1009E24.1; SN; Siglec-1
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ClonalityMonoclonal
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Immunoglobulin isotypeIgG1
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Clone7-239
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Host organismMouse (Mus musculus)
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Species reactivityHuman (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityCD169 Recognises human CD169, otherwise known as Sialoadhesin (Sn), a type I transmembrane glycoprotein and prototypic member of the Siglec (sialic acid binding Ig-like lectin) family, designated Siglec-1. _x000D__x000D_CD169 is a macrophage-restricted interaction molecule which binds to sialylated ligands on haematopoietic cells, including neutrophils, NK cells, monocytes, a subset of CD8+ T cells and B cells, through recognition of sialic acid in the alpha-2,3- glycosidic linkage. CD169 is highly expressed by dendritic cells and on stromal macrophages in the spleen, bone marrow and lymph nodes and to a lesser extent in liver, lungs and gut, and studies have shown high expression of CD169 on macrophages in the inflammatory conditions rheumatoid arthritis and atherosclerosis. _x000D__x000D_Clone 7-239 completely inhibits erythrocyte rosetting around Sn+ transductants.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form AppearanceFITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
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ConcentrationIgG concentration 0.1 miligram per 1 mililiter
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Storage and shippingStore the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applicationsFlow Cytometry (FACS)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestMBS Monoclonals supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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ConjugationAnti-FITC Antibody
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolSIGLEC1
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Short nameMOUSE Anti- CD169:FITC Antibody
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Techniqueanti-Human, Antibody, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, Fluorescein, mouses
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Hostmouse
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameAnti-MOUSE ANTI HUMAN CD169:FITC
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Alternative techniqueantibodies, murine, fluorescine
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Gene info
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Identity
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Gene
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Long gene namesialic acid binding Ig like lectin 1
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Synonyms gene
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Synonyms gene name
- sialoadhesin
- sialic acid binding Ig-like lectin 1, sialoadhesin
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-03-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- C2-set domain containing
- I-set domain containing
- Sialic acid binding Ig like lectins
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data