Mouse IgM-FITC
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Catalog numberGEN666583
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Price:
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Size100ug
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Also known asIgM
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Other namesIgM, partial; N/A
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameIgM
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Gene name synonimsN/A
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Other gene namesN/A
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ClonalityMonoclonal
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Immunoglobulin isotypeIgMk
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CloneTEPC183
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Host organismMouse (Mus musculus)
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Species reactivityN/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityN/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form Appearance50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
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Concentration1.0 mg/ml
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Storage and shippingStore the antibody at +4°C. Do NOT freeze the antibody. the antibody is photosensitive and should not be exposed to direct bright light. If stored under proper conditions, the activity of the antibody will be retained for at least one year.
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Tested applicationsFlow Cytometry (FC/FACS), Immunohistochemistry (IHC), ELISA (EIA)
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PropertiesThis MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target-
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Short nameMouse IgM-FITC
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TechniqueMouse, FITC, IgM, Fluorescein, IgMs, mouses
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Hostmouse
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IsotypeIgM, IgM
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameAnti-Mouse IgM-FITC
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Alternative techniquemurine, fluorescine, immunoglobulin-m
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MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data