Maltose Phosphorylase (Biotin)
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Catalog number
MBS615292
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Price
Please ask
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Size
0.1 mg
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Products_type
Antibody
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Products_short_name
[Maltose Phosphorylase]
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Products_name_syn
[Anti -Maltose Phosphorylase (Biotin)]
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Other_names
[maltose phosphorylase (EC 2.4.1.8) - Bacillus sp. (Strain RK-1)]
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Clonality
Polyclonal
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Reactivity
Goat
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Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Maltose Phosphorylase (E.coli).
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Purity
Highly Purified Purified by delipidation, salt fractionation and ion exchange chromatography.
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Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
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Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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