Hygromycin B Solution w/ 100mg/ml Hygromycin B in 25mM HEPES buffer, pH 7.0

• Catalog number
  A034-50ML
• Price
  Please ask
• Size
  50ML

• Description
  Hygromycin B Solution, w/ 100mg/ml Hygromycin B in 25mM HEPES buffer, pH 7.0, is a brown coloured clear solution. This antibiotic kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
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• Shelf life
  Use before expiry date given on the product label.
• Storage
  store between 2-8°C
• Appearance
  Brown coloured clear solution
• Uses
  Laboratory chemicals, Manufacture of substances
• Personal Protection
  Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
• Technical Data
  Contact us to request a Technical Data
• Data sheet
• Ordering
  To order Hygromycin B Solution w/ 100mg/ml Hygromycin B in 25mM HEPES buffer, pH 7.0, please use the Cat. Nr.A034-50ML and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for Hygromycin B Solution w/ 100mg/ml Hygromycin B in 25mM HEPES buffer, pH 7.0
• Additional description
  Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

• Gene target
  Hygromycin   100mg   Hygromycin   25mM  
• Short name
  Hygromycin B w/ 100mg/ Hygromycin B in 25mM , pH 7
• Alternative name
  Hygromycin B solution w/ 100mg/milliliter Hygromycin B in 25mM HEPES solution, pH 7.0

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