RPMI-1640 w/ 25mM HEPES buffer and Sodium pyruvate
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Catalog number
AT222A-10X1LX3
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Price
Please ask
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Size
3X10X1L
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Description
RPMI-1640 w/ 25mM HEPES buffer and Sodium pyruvate w/o Glucose, L-Glutamine and Sodium bicarbonate is a White to light pink, homogenous powder,Produced by HiMedia Laboratories Private Limited
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Storage conditions
store between 2-8°C
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Appearance
White to light pink, homogenous powder
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Solubility
Soluble in water
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Use
used in laboratory chemicals, Manufacture of substances
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Technical data
Please contact us to request the technical data
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Additional description
Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia.
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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