Human CellExp™ TIGIT, human recombinant
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Catalog number
8015-10
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Price
Please ask
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Size
10 µg
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Synonyms
TIGIT, VSIG9, VSTM3
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Alternative_names
TIGIT, VSIG9, VSTM3
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Description
>95% Pure, Human cell expressed TIGIT
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per ug of the protein by the LAL method.
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Molecular Weight
This protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 15 kDa. The predicted N-terminus is Met 22. DTT-reduced Protein migrates as 18-24 kDa in SDS-PAGE due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
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Background Information
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
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Amino acid sequence
Met 22 – Pro 141
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TIGIT
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Short name
CellExp™ TIGIT, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ T cellular immunoreceptor including Ig and ITIM domains, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
T cell immunoreceptor with Ig and ITIM domains, TIGIT and IDBG-50851 and ENSG00000181847 and 201633, protein binding, Cell surfaces, Tigit and IDBG-161101 and ENSMUSG00000071552 and 100043314, TIGIT and IDBG-632631 and ENSBTAG00000011921 and 100140801
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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