Hrk Antibody
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Catalog number3176-100
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PricePlease ask
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Size100 µg
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Antibody TargetHrk
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Target Alternative NameBID3
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceSynthetic peptide corresponding to residues surrounding amino acids 50 of human HRK
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AccessionAAC34931
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Gene Id8739
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) antigen affinity purified rabbit anti-human Hrk polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blot analysis (1 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects human Hrk fused with green fluorescent protein in crude extracts of E. coli transfected with Hrk-GFP.
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DescriptionMembers of the Bcl-2 family of proteins interact to regulate programmed cell death, or apoptosis. Various homodimers and heterodimers formed by proteins in this family can either promote or inhibit apoptosis. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect on certain hematopoietic cell lines following growth factor withdrawal. Additional apoptotic inhibitors in this family include Bcl-x, Bcl-w, Mcl-1, Bag-1 and A1. Pro-apoptotic members of this family include Bax, Bad, Bak, NBK (Bik), BID and Hrk. Hrk (for harakiri), designated DP5 or neuronal death protein in mouse and rat, contains a BH3 domain with high homology to other Bcl-2 family members but lacks the conserved BH1 and BH2 domains. Physical interaction of Hrk with Bcl-2 or Bcl-xL inhibits the apoptotic activity of Hrk.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolHRK
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Short nameHrk Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameharakiri, BCL2 interacting protein (contains only BH3 domain) (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetharakiri, BCL2 interacting protein (contains only BH3 domain), DP5 and HARAKIRI, HRK and IDBG-59297 and ENSG00000135116 and 8739, protein binding, Plasma membranes, Hrk and IDBG-195140 and ENSMUSG00000046607 and 12123, HRK and IDBG-633660 and ENSBTAG00000047484 and 787139
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Gene info
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Identity
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Gene
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Long gene nameharakiri, BCL2 interacting protein
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Synonyms gene name
- harakiri, BCL2-interacting protein (contains only BH3 domain)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-05-21
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Entrez gene record
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Pubmed identfication
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Classification
- BCL2 homology region 3 (BH3) only
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data