Hamster IgG Isotype Control (FITC)
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Catalog number
abx016616
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Price
Please ask
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Size
0.2 mg
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Category
Isotype Control
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Clonality
Polyclonal
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Raised in
Hamster
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Target
Hamster IgG Isotype Control
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Immunogen
see included datasheet or contact us
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Species reactivity
Human, Mouse, Rat (for other species please inquire)
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Purification
Affinity purified
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Applications
WB (for other applications please contact us)
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Stock availability
Shipped within 5-10 working days.
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Storage conditions
Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
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Footnote
This product is for research use only.
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
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Properties
This Abbexa Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Abbexa FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Group
isotypes
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Short name
IgG Isotype Control (FITC)
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Technique
Control, Isotype, FITC, IgG, controls, Fluorescein, IgGs, Isotypes and controls
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Host
Hamster
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Isotype
IgG, IgG
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Label
FITC
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Species
Hamster, Hamsters
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Alternative name
Hamster Immunoglobulin G isoform reference (fluorecein)
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Alternative technique
control, controls, fluorescine, immunoglobulins
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Tissue
control
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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