DMEM - F12 w/o L-Glutamine w/ 25 mM Hepes - 500ml
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Catalog number
LM-D1226/500
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Price
Please ask
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Size
25X500ml
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Description
DMEM - F12 w/o L-Glutamine w/ 25 mM Hepes - 500ml is used in the maintenance and the culturing of mammalian cells. It offers excellent performance for certain epithelial, endothelial and granulose cell types.
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Shipping conditions
-20°C or dry ice
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Storage
+2/+8°C
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Shelflife
24 months
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State
Liquid
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Chemical description
Water-based mixture of inorganic and organic compounds
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Appearance
Physical state at 20 ºC: Liquid ; Appearance: Semitransparent ; Color: Red
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Density at 20 ºC
1040 kg/m³
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Relative density at 20 ºC
1.04
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PH
7.2 ± 0.3
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Osmolality
304 mOsm/kg ±10 %
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Cell growth test
Medium tested for the ability to support cell growth with Hela line.
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Composition
NA
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Sterility
Sterile filtered
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Personal precautions
Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
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Uses
Usage biotechnologique. Uniquement pour usage professionnel.
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Ordering
To order DMEM - F12 w/o L-Glutamine w/ 25 mM Hepes - 500ml, please use the Cat. Nr.LM-D1226/500 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for DMEM - F12 w/o L-Glutamine w/ 25 mM Hepes - 500ml
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
Gene info
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Identity
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Gene
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Long gene name
coagulation factor XII
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Synonyms gene name
- coagulation factor XII (Hageman factor)
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GenBank acession
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
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Locus Specific Databases
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