DMEM F12 w/o LGlutamine w/ 25 mM Hepes
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Catalog numberLM-D1226/500
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Price:
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Size500ml
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Ordering informationIf you wish to order this product, please send us by email or by fax an official purchase order including your full shipping and billing information. Contact us if you need first a quotation. Delivery for USA and Europe next week if an order is received before Friday 2 pm.
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Technical datasheetPlease contact us to receive the technical datasheet or also the MSDS file
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ContactCall us at +32 16 58 90 45 for more technical information about this product
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Buffer25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Gene target
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Gene symbolF12
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Short nameDMEM F12 w/o LGlutamine w/ 25 mM
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Alternative nameDMEM F12 w/o LGlutamine w/ 25 millimeter Hepes
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Gene info
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Identity
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Gene
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Long gene namecoagulation factor XII
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Synonyms gene name
- coagulation factor XII (Hageman factor)
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
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Locus Specific Databases