GSTM5, human recombinant

• Catalog number
  7802-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Glutathione S-transferase mu 5, GST5, GSTM5-5
• Alternative_names
  Glutathione S-transferase mu 5, GST5, GSTM5-5
• Description
  Functions in the detoxification of electrophilic compounds
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity > 40 units/mg, a unit is defined as the amount of enzyme that conjugate 1.0 µmole of 1-chloro-2, 4-dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25ºC.
• Results
  Specific activity > 40 units/mg
• Unit Definition
  One unit is defined as the amount of enzyme that conjugate 1.0 µmole of 1-chloro-2, 4-dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25ºC.
• Molecular Weight
  28.2 kDa (242 aa, 1-218 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol, 1 mM DTT and 100 mM NaCl.
• Background Information
  Glutathione S-transferase mu 5, also known as GSTM5, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. GSTM5 plays an important role in detoxification. Recombinant human GSTM5 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSHMPMTLG YWDIRGLAHA IRLLLEYTDS SYVEKKYTLG DAPDYDRSQW LNEKFKLGLD FPNLPYLIDG AHKITQSNAI LRYIARKHNL CGETEEEKIR VDILENQVMD NHMELVRLCY DPDFEKLKPK YLEELPEKLK LYSEFLGKRP WFAGDKITFV DFLAYDVLDM KRIFEPKCLD AFLNLKDFIS RFEGLKKISA YMKSSQFLRG LLFGKSATWN SK
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GSTM5  
• Gene symbol
  GSTM5
• Short name
  GSTM5, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  GSTM5, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4637
• Gene
  GSTM5
• Long gene name
  glutathione S-transferase mu 5
• Synonyms gene name
  • glutathione S-transferase M5
• GenBank acession
  • L02321
• Locus
  1p13.3
• Discovery year
  1993-06-15
• Entrez gene record
  2949
• Pubmed identfication
  • 8473333
• RefSeq identity
  • NM_000851
• Classification
  • Soluble glutathione S-transferases
• VEGA ID
  OTTHUMG00000011644

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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