Donkey anti Goat IgG (heavy and light chains), Secondary Antibodies

• Catalog number
  DoAG-IgG(H+L)-7S
• Price
  Please ask
• Size
  10mg

• Host Source
  Donkey
• Clone Name
  Polyclonal
• Product Category
  Veterinary
• Species Reactivity
  Caprine
• Application
  Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
• Immunogen
  Purified normal IgG isolated from pooled goat serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
• Purification Method
  NA
• Shipping Conditions
  NA
• Storage conditions
  Donkey anti Goat IgG (heavy and light chains), Secondary Antibodies philized IgG fraction is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days
• Datasheet link
  NA
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Latin name
  Equus africanus asinus, Capra aegagrus hircus
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Gene target
  heavy   light   chains   Secondary   Antibodies  
• Short name
  anti Goat IgG (heavy light chains), Secondary Antibodies
• Technique
  light, anti, Goat, IgG, antibody to, goats, IgGs
• Host
  Goat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  NA, IgG
• Species
  Donkeys, Goats
• Alternative name
  Donkey antibody to caprine Immunoglobulin G (heavy and light chains), secondary detector Antibody Antibodies
• Alternative technique
  antibodies, caprine, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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