Goat anti Human Ig lambda light chains (free and bound), conjugated with Biotin, Secondary Antibodies

• Catalog number
  SR-GAHu-BJL(SD+HD)-Bio
• Price
  Please ask
• Size
  1ml

• Host Source
  Goat
• Clone Name
  Polyclonal
• Product Category
  Immunology
• Species Reactivity
  Human
• Application
  ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
• Immunogen
  A pool of purified Bence Jones lambda proteins isolated from human urine. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
• Purification Method
  NA
• Shipping Conditions
  NA
• Storage conditions
  Goat anti Human Ig lambda light chains (free and bound), conjugated with Biotin, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4ÁC--at least 10 years. Reconstituted at or below -20ÁC--3-5 years. Reconstituted at +4ÁC--7 days.
• Datasheet link
  NA
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Biotinylated
• Latin name
  Capra aegagrus hircus

• Gene target
  lambda   light   chains   free   bound   conjugated   with   Secondary   Antibodies  
• Short name
  Goat anti Ig lambda light chains (free bound), conjugated with , Secondary Antibodies
• Technique
  anti human, light, anti, Goat, antibody to, anti human antibodies, antibody Conjugates, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  NA
• Label
  Biotin
• Species
  Human, Goats, Humans
• Alternative name
  caprine antibody to H. sapiens Ig lambda light chains (free and bound), coupled including biotinilated, secondary detector Antibody Antibodies
• Alternative technique
  antibodies, caprine

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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