Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric)

• Catalog number
  K935-100
• Price
  Please ask
• Size
  100 assays

• Description
  Sensitive Assay, HTS
• Summary
  • Detection method- Absorbance (450 nm) • Applications- - Measurement of sorbitol dehydrogenase (SDH) activity in various tissues/cells - • Analysis of polyol-pathways
• Detection Method
  Absorbance (450 nm)
• Species Reactivity
  Eukaryotes
• Applications
  • Measurement of Sorbitol Dehydrogenase Activity in various tissues/cells. • Analysis of polyol-pathway. • Studies of chemically-induced hepatotoxicity and acute liver injury. • Freshly obtained serum from animals with suspected liver damage
• Sample Type
  • Animal tissues: Liver, Kidney etc. • Cell culture: HeLa, Jurkat etc.
• Features Benefits
  • Simple, rapid & convenient • Can measure SDH activity as low as 50 µU in a variety of samples
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Sorbitol Dehydrogenase (SDH), also referred to as L-iditol dehydrogenase (EC 1.1.1.14) participates in the polyol pathway. Also known as the sorbitol-aldose pathway, this pathway is divided into two steps: first, glucose is reduced to sorbitol via the catalytic action of aldose reductase (AR), while the second step is catalyzed by sorbitol dehydrogenase, which utilizes sorbitol as substrate and subsequently produces fructose. Sorbitol is not a cell membrane permeable molecule; whereas fructose can be further metabolized inside the cells. Recent studies found that deficiency of Sorbitol Dehydrogenase causes the intracellular accumulation of sorbitol which leads complications in diabetes. The enzyme is predominately expressed in the liver cells and is only released into the bloodstream following acute liver damage. Therefore, the presence of significant SDH activity in serum is a potential indicator of liver injury or disease. BioVision’s SDH assay kit provides a quick and easy way for monitoring SDH activity in various samples. In this Assay, Sorbitol Dehydrogenase utilizes a provided substrate while reducing NAD+ to form NADH. NADH reacts with the developer, leading to the formation of a chromophore with strong absorbance at OD 450 nm. The assay is simple, sensitive and can detect Sorbitol Dehydrogenase Activity less than 50 µU in variety of samples
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Sorbitol   Dehydrogenase   Activity   Kit  
• Short name
  Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Sorbitol Dehydrogenase Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products