Detection limits 46.88-3000 pg/mL
Sensitivity limit 1,64 pg/mL
Samples to be analyzed Serum, plasma and other biological fluids
Assay duration 2 hours, 40min
Verified applications CLIA
ELISA detection Chemiluminescent
Protein number Please refer to Uniprot
Gene number Please refer to GenBank
Gene name Fibroblast Growth Factor 1, Acidic
Alternate gene name ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 1, Acidic (FGF1). No significant cross-reactivity or interference between Fibroblast Growth Factor 1, Acidic (FGF1) and analogues was observed.
Assay principle The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 1, Acidic (FGF1) level in the sample or standard.;
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
Warnings Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Properties Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
Description Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Short name Fibroblast Growth Factor 1, Acidic (FGF1) CLIA Kit, 96
Alternative name Chicken Fibroblast Growth Factor 1, Acidic (fibroblast growth factor 1 (acidic)) CLIA reagent, 96 well plate
Alternative technique kits
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832, fibroblast growth factor 1 (acidic), AFGF and ECGF and ECGF-beta and ECGFA and ECGFB and FGF-1 and FGF-alpha and FGFA and GLIO703 and HBGF-1 and HBGF1, FGF1 and IDBG-51235 and ENSG00000113578 and 2246, S100 protein binding, nuclei, Fgf1 and IDBG-141787 and ENSMUSG00000036585 and 14164, FGF1 and IDBG-646137 and ENSBTAG00000005198 and 281160