- Catalog numberEKU09631
- Product namePorcine Fibroblast Growth Factor 1, Acidic (FGF1) CLIA Kit, 96 well plate
- Size10x96T
- PriceAsk For Price
- Verified reactivitySus scrofa; Porcine (Pig)
- Detection limits62.50-4000 pg/mL
- Sensitivity limit2,41 pg/mL
- Samples to be analyzedSerum, plasma and other biological fluids
- Assay duration2 hours, 40min
- Verified applicationsCLIA
- Assay classSandwich
- ELISA detectionChemiluminescent
- Protein numberP20002
- Alternate protein numberPlease refer to SwissProt
- Gene numberPlease refer to GenBank
- Gene nameFibroblast Growth Factor 1, Acidic
- Alternate gene nameECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta
- Estimated production time7-11 business days
- Shipping requirementsBlue ice
- Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Use for8 months
- ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 1, Acidic (FGF1). No significant cross-reactivity or interference between Fibroblast Growth Factor 1, Acidic (FGF1) and analogues was observed.
- ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
- Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 1, Acidic (FGF1) level in the sample or standard.;
- ProtocolPlease see ELISA's datasheet, otherwise contact us
- Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
- ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
- Research main areaCytokine;Tumor immunity;Infection immunity;
- WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- NotesFor research use only. Not for diagnostic procedures.
- Hyperlink
- Alternative to gene target
- [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
- [ "fibroblast growth factor 1 (acidic)", "AFGF and ECGF and ECGF-beta and ECGFA and ECGFB and FGF-1 and FGF-alpha and FGFA and GLIO703 and HBGF-1 and HBGF1", "FGF1 and IDBG-51235 and ENSG00000113578 and 2246", "S100 protein binding", "nuclei", "Fgf1 and IDBG-141787 and ENSMUSG00000036585 and 14164", "FGF1 and IDBG-646137 and ENSBTAG00000005198 and 281160" ]
- Gene targetFibroblast Growth Factor 1 Acidic FGF1 CLIA Kit 96
- Identity:HGNC:3665
- Gene:FGF1
- Long gene name:fibroblast growth factor 1
- Synonyms gene name:fibroblast growth factor 1 (acidic)
- Synonyms:AFGFECGFECGFAECGFBHBGF1ECGF-betaFGF-alphaGLIO703
- Discovery year:1986-01-01
- Entrez gene record:2246
- Pubmed identification:1697263, 3523756
- RefSeq identity:NM_000800
- Classification:Fibroblast growth factor family, Receptor ligands
- VEGA ID:OTTHUMG00000059703
- Gene symbolFGF1
- Short namePorcine Fibroblast Growth Factor 1, Acidic (FGF1) CLIA Kit, 96
- Alternative techniquekits