Calpain-2 Antibody
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Catalog number3372-100
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PricePlease ask
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Size100 µl
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Antibody TargetCalpain 2
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Target Alternative NameM-Calpain , Calpain 2 , Calpian II , CANPL2 , CANP2 , millimolar-calpain , mCANP , FLJ39928 , CANPml , EC 3.4.22.53
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide mapping to the C-terminus of the domain III of human Calpain 2 large subunit
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AccessionNP_001739
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Gene Id824
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µl affinity purified rabbit polyclonal anti-calpain 2 antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blotting (1:200-1000 dilutions). However, the optimal concentrations should be determined individually. The anti-calpain 2 antibody recognizes the 80 kDa Calpain 2. It does not cross-react with other calpains
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DescriptionCalpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF-hand domain IV. Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolCAPN8, CAPN2, CAPNS2
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Short nameCalpain-2 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameCalpain-2 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namecalpain 8
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Synonyms gene name
- calpain 8 (nCL-2)
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Synonyms
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Locus
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Discovery year1998-09-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Calpains
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecalpain 2
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Synonyms gene name
- calpain 2, (m/II) large subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- EF-hand domain containing
- Calpains
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecalpain small subunit 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-07-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- EF-hand domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data