mgMT Antibody
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Catalog number3820-30T
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PricePlease ask
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Size30 µg
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Antibody TargetmgMT
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Target Alternative NameO-6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, Methylguanine DNA methyltransferase , Methylated DNA protein cysteine methyltransferase
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Antibody TypePolyclonal
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Species ReactivitiesMouse, rat
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Immunogen SequenceSynthetic peptide corresponding to residues surrounding amino acids 8 of rat mgMT
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AccessionP24528
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Gene Id25332
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation30 µg (0.5 mg/ml) affinity purified rabbit anti- mgMT polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
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DescriptionmgMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair protein that response to DNA damage by removing methly or alkyl groups from the O6 position of guanine. mgMT protects normal cells from cytotoxic effects and is important in drμg resistance to alkylating agents.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMGMT
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Short namemgMT Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameO-6-methylguanine-Desoxyribonucleic acid methyltransferase (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetO-6-methylguanine-DNA methyltransferase, MGMT and IDBG-93188 and ENSG00000170430 and 4255, DNA-methyltransferase activity, nuclei, Mgmt and IDBG-211408 and ENSMUSG00000054612 and 17314
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Gene info
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Identity
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Gene
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Long gene nameO-6-methylguanine-DNA methyltransferase
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-10-16
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Entrez gene record
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RefSeq identity
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Classification
- Methyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data