Anti-HLA B27 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-8604R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  HLA B27
• Specificity
  This antibody reacts specifically with HLA B27
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Extracellular
• Antigen Source
  KLH conjugated synthetic peptide derived from human HLA B27
• Gene ID
  N/A
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind peptides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-B encodes a membrane anchored heavy chain which hetero-dimerizes with a light chain (∫-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-B alleles. The HLA-B27 allele appears with increased frequency in uveitis patients.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  HLA B27 HLA-B27; HLAB; Leukocyte antigen class I B; Lymphocyte antigen; Major histocompatibility complex class I B; SPDA1; 1B27_HUMAN.
• Also known as
  HLA B27 Polyclonal Antibody
• Other name
  Anti-HLA B27 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  HLA   B27  
• Gene symbol
  HLA-DPA2, HLA-DOA, HLA-DQA1, HLA-N, HLA-DQA2, HLA-DPB1, HLA-DQB2, HLA-W, HLA-DRA, HLA-S
• Short name
  Anti-HLA B27
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  HLA B27 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:4939
• Gene
  HLA-DPA2
• Long gene name
  major histocompatibility complex, class II, DP alpha 2 (pseudogene)
• Synonyms gene
  • HLA-DP2A
• GenBank acession
  • X01634
• Locus
  6p21.32
• Discovery year
  1986-01-01
• Entrez gene record
  646702
• Pubmed identfication
  • 6597088
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031061
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4936
• Gene
  HLA-DOA
• Long gene name
  major histocompatibility complex, class II, DO alpha
• Synonyms gene
  • HLA-DZA
  • HLA-DNA
• Synonyms
  • HLA-D0-alpha
• GenBank acession
  • M31525
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3111
• Pubmed identfication
  • 2370084
• RefSeq identity
  • NM_002119
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031211
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4942
• Gene
  HLA-DQA1
• Long gene name
  major histocompatibility complex, class II, DQ alpha 1
• Synonyms gene
  • HLA-DQA
• Synonyms
  • CELIAC1
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3117
• RefSeq identity
  • NM_002122
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031106
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:19406
• Gene
  HLA-N
• Long gene name
  major histocompatibility complex, class I, N (pseudogene)
• Synonyms
  • HLA-30
• GenBank acession
  • M96329
• Locus
  6p22.1
• Discovery year
  2002-10-23
• Entrez gene record
  267014
• Pubmed identfication
  • 10452889
  • 1517563
• RefSeq identity
  • NG_009940
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031167
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4943
• Gene
  HLA-DQA2
• Long gene name
  major histocompatibility complex, class II, DQ alpha 2
• Synonyms gene
  • HLA-DXA
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3118
• RefSeq identity
  • NM_020056
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031108
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4940
• Gene
  HLA-DPB1
• Long gene name
  major histocompatibility complex, class II, DP beta 1
• Synonyms gene
  • HLA-DP1B
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3115
• RefSeq identity
  • NM_002121
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031076
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4945
• Gene
  HLA-DQB2
• Long gene name
  major histocompatibility complex, class II, DQ beta 2
• Synonyms gene
  • HLA-DXB
• GenBank acession
  • M83890
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3120
• Pubmed identfication
  • 2564844
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031125
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:23425
• Gene
  HLA-W
• Long gene name
  major histocompatibility complex, class I, W (pseudogene)
• Synonyms gene
  • HLA-80
• Synonyms gene name
  • HLA-80 pseudogene
• GenBank acession
  • M96334
• Locus
  6p22.1
• Discovery year
  2004-01-16
• Entrez gene record
  352966
• Pubmed identfication
  • 10557312
  • 1517563
• RefSeq identity
  • NG_015995
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031242
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4947
• Gene
  HLA-DRA
• Long gene name
  major histocompatibility complex, class II, DR alpha
• Synonyms gene
  • HLA-DRA1
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3122
• RefSeq identity
  • NM_019111
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031269
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:19395
• Gene
  HLA-S
• Long gene name
  major histocompatibility complex, class I, S (pseudogene)
• Synonyms
  • HLA-17
• GenBank acession
  • M96339
• Locus
  6p21.33
• Discovery year
  2002-10-23
• Entrez gene record
  267015
• Pubmed identfication
  • 10452889
  • 1517563
• RefSeq identity
  • NG_009941
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031178
• Locus Specific Databases
  • IMGT/HLA Database

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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