CXCR4 Antibody
-
Catalog number5224-100
-
PricePlease ask
-
Size100 µg
-
-
Antibody TargetCXCR4
-
Target Alternative Namechemokine (C-X-C motif) receptor 4, CXC chemokine receptor 4, CXC chemokine receptor -4, CXC chemokine receptor
-
Antibody TypePolyclonal
-
Species ReactivitiesHuman, mouse
-
Immunogen SequenceSynthetic peptide mapping to the N-terminus of human CXCR4
-
AccessionP61073
-
Gene Id7852
-
AppearanceColorless liquid
-
FormLiquid
-
Concentration0.5 mg/ml
-
Formulation100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-CXCR4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
-
PurificationAffinity purified
-
ApplicationWestern blot, Immunoprecipitation, Immunohistochemistry
-
Application And UsagesWestern blot analysis (0.5-4 µg/ml), immunoprecipitation, and immunocytochemistry. However, the optimal conditions should be determined individually. A 43 kDa band should be detected. HeLa cell lysate can be used as a positive control.
-
DescriptionHuman immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The α-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.
-
Storage Condition-20 °C
-
Shipping Conditiongel pack
-
Shelf Life12 months
-
UsageFor Research Use Only! Not to be used in humans.
-
HandlingThe antibody solution should be gently mixed before use.
-
Western Blot VerifiedVerified
-
Immunocytochemistry VerifiedNot Verified
-
Immunofluorescence VerifiedNot Verified
-
Immunoprecipitation VerifiedNot Verified
-
FACS VerifiedNot Verified
-
ELISA VerifiedNot Verified
-
ChIP VerifiedNot Verified
-
Flow Cytometry VerifiedNot Verified
-
PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolCXCR4
-
Short nameCXCR4 Antibody
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit IgG
-
Alternative namechemokine (C-X-C motif) receptor 4 (antibody to-)
-
Alternative techniqueantibodies
-
Alternative to gene targetchemokine (C-X-C motif) receptor 4, CD184 and D2S201E and FB22 and HM89 and HSY3RR and LAP-3 and LAP3 and LCR1 and LESTR and NPY3R and NPYR and NPYRL and NPYY3R and WHIM, CXCR4 and IDBG-71032 and ENSG00000121966 and 7852, ubiquitin binding, Cell surfaces, Cxcr4 and IDBG-189999 and ENSMUSG00000045382 and 12767, CXCR4 and IDBG-642187 and ENSBTAG00000001060 and 281736
-
Gene info
-
Identity
-
Gene
-
Long gene nameC-X-C motif chemokine receptor 4
-
Synonyms gene name
- chemokine (C-X-C motif), receptor 4 (fusin)
- chemokine (C-X-C motif) receptor 4
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1998-09-17
-
Entrez gene record
-
Pubmed identfication
-
Classification
- C-X-C motif chemokine receptors
- CD molecules
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data