AKT1, potassium channel AKT1

#

• Catalog number
  AS09 516
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• Size
  200 µg

# #

• Available ordering format
  Lyophilized
• Immunogen
  KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified serum
• How to reconstitute
  For reconstitution add 100 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  AS09 515 | anti-KC1 | potassium channel KAT3collection of antibodies to membrane transport systemPlant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 50 with 125I (WB)
• Molecular weight expected аpparent
  96.9 kDa
• Verified reactivity
  Arabidopsis thaliana
• Possible reactivity
  Arabidopsis thaliana
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
• References
  Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047. Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.
• Scientific context
  AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
• Notes
  for detection images please, refer to the publication belowAntibody detects native and recombinant AKT1.
• Protein number
  Q38998
• TAIR number
  AT2G26650
• Hyperlink

#

• Gene target
  AKT1   potassium   channel   AKT1  
• Gene symbol
  AKT1
• Short name
  AKT1, potassium channel AKT1
• Alternative name
  v-akt murine thymoma viral oncogene homolog 1, potassium channel v-akt murine thymoma viral oncogene homolog 1
• Alternative to gene target
  v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and

Gene info

• Identity
  HGNC:391
• Gene
  AKT1
• Long gene name
  AKT serine/threonine kinase 1
• Synonyms gene name
  • v-akt murine thymoma viral oncogene homolog 1
• Synonyms
  • RAC
  • PKB
  • PRKBA
  • AKT
  • RAC-alpha
• Synonyms name
  • protein kinase B
• GenBank acession
  • M63167
• Locus
  14q32.33
• Discovery year
  1986-01-01
• Entrez gene record
  207
• Pubmed identfication
  • 21214427
• RefSeq identity
  • NM_005163
• Classification
  • Pleckstrin homology domain containing
  • AKT kinases
• VEGA ID
  OTTHUMG00000170795
• Locus Specific Databases
  • LRG_721

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