Active NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP)
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Catalog number
7605-200
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Price
Please ask
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Size
200 ug
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Synonyms
primary alcohol dehydrogenase; ADH; aliphatic alcohol dehydrogenase; ethanol dehydrogenase; NAD-specific aromatic alcohol dehydrogenase; NADP-aldehyde dehydrogenase; yeast alcohol dehydrogenase; alcohol dehydrogenase activity; aldehyde dehydrogenase (NADP) activity
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Alternative_names
primary alcohol dehydrogenase; ADH; aliphatic alcohol dehydrogenase; ethanol dehydrogenase; NAD-specific aromatic alcohol dehydrogenase; NADP-aldehyde dehydrogenase; yeast alcohol dehydrogenase; alcohol dehydrogenase activity; aldehyde dehydrogenase (NADP) activity
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Description
A cyclic pentapeptide vascular-targeting drug.
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
TThis NADP-dependent isopropanol dehydrogenase has biological activity ≥60U/mg based on the following reaction using BioVision kit (#K698-100): Isopropanol + NADP+ Acetone + NADPH BioVision’s NADP/NADPH quantification colorimetric kit (#K347-100) can also be used to test the activity of NADP-dependent isopropanol dehydrogenase.
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Unit Definition
One unit is the amount of enzyme that will generate 1.0 µmole of NADPH per minute at pH 8 at 37 ̊C.
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Molecular Weight
~40.9 kDa (SDS-PAGE)
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Storage Temp
-20°C
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Shipping
gel pack
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Shelf Life
12 months
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Appearance
Liquid
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Physical form description
1 mg/mL solution in 50 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl and 50% glycerol
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Background Information
Alcohol dehydrogenase with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Has very low activity with primary alcohols, such as ethanol. Under physiological conditions, the enzyme reduces aldehydes and 2-ketones to produce secondary alcohols. Is also active with acetaldehyde and propionaldehyde
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Gene info
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