A12.2, RNA polymerase I subunit (homolog of Pol II Rpb9)

• Catalog number
  AS07 225
• Price
  Please ask
• Size
  200 µg

• Available ordering format
  Lyophilized in PBS pH 7.4
• Immunogen
  KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified serum
• How to reconstitute
  For reconstitution add 143 µl of sterile water
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  Plant protein extraction buffer Secondary antibodies
• Recommended dilutions for use
  1 : 2 000 with standard ECL (WB)
• Molecular weight expected аpparent
  13.6 kDa
• Verified reactivity
  Arabidopsis thaliana
• Possible reactivity
  Arabidopsis thaliana
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  This antibody is specific for A12.2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana
• References
  to be added when available
• Scientific context
  RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription. In yeast subunit A12.2 has been described as homologous to the Rpb9 subunit from polymerase II.
• Notes
  This product has previously been labelled as anti-At3g25940 transcription factor S-II (TFIIS) domain-containing protein.Protocol for isolation of cytosolic and nuclear fractions can be found here.
• Protein number
  Q9LU97
• TAIR number
  AT3G25940
• Hyperlink

• Gene target
  A12   RNA   polymerase   subunit   homolog   Pol   Rpb9  
• Gene symbol
  SNAR-A12, PCDHGA12
• Short name
  A12 2, RNA polymerase I subunit (homolog of Pol II Rpb9)
• Technique
  RNAs are to store at -70 C
• Alternative name
  A12.2, RNA polymerizing enzyme I functionnal sequence (homolog on Pol II Rpb9)

Gene info

• Identity
  HGNC:34315
• Gene
  SNAR-A12
• Long gene name
  small NF90 (ILF3) associated RNA A12
• Synonyms gene name
  • small nuclear ILF3/NF90-associated RNA A12
  • small ILF3/NF90-associated RNA A12
• GenBank acession
  • EU071062
• Locus
  19q13.33
• Discovery year
  2008-06-20
• Entrez gene record
  100126800
• Pubmed identfication
  • 17855395
• RefSeq identity
  • NR_004437
• Classification
  • Small NF90 (ILF3) associated RNAs

Gene info

• Identity
  HGNC:8699
• Gene
  PCDHGA12
• Long gene name
  protocadherin gamma subfamily A, 12
• Synonyms gene
  • CDH21
• Synonyms gene name
  • cadherin 21
• Synonyms
  • KIAA0588
  • FIB3
  • PCDH-GAMMA-A12
• Synonyms name
  • fibroblast cadherin FIB3
• GenBank acession
  • AF152506
• Locus
  5q31.3
• Discovery year
  2000-06-28
• Entrez gene record
  26025
• Pubmed identfication
  • 10380929
• RefSeq identity
  • NM_003735
• Classification
  • Clustered protocadherins
• VEGA ID
  OTTHUMG00000129611

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