Human POU5F1 (Oct4) Recombinant Protein

• Catalog number
  S2105-20UG
• Price
  Please ask
• Size
  20 ug

• Storage conditions
  Store at -80 °C. Avoid multiple freeze-thaws.
• Shipping conditions
  dry ice
• Shelf life
  12 months
• Components
  300 µg/ml (determined by A280) in hs-PBS (1.5 mM KH2PO4, 8.1 mM Na2HPO4, 500 mM NaCl, 2.7 mM KCl, pH 7.4) + 5% glycerol
• Applications
  SDS-PAGE, WB, ELISA, Dot
• Features
  Amino acids 1-212; Protein size and MW: 23 kDa (AA 1-212). Full length POU5F1 = 38.6 kDa (AA 1-360); Purity: >90% by SDS-PAGE
• Description
  Recombinant Protein expressed in E. coli; POU5F1, commonly known as Octamer-binding protein 4 (Oct4), is a POU transcription factor family member that is critical for maintaining embryonic stem (ES) cell pluripotency and preventing differentiation to germ layers and mature cell lineages. For this reason, POU5F1 expression is frequently used as a marker for undifferentiated stem cells. It contains a POU-specific domain and a homeobox DNA-binding domain. When transfected together with other key pluripotent transcription factors, POU5F1 has been used to reprogram somatic cells into induced pluripotent stem (iPS) cells.
• Product type
  Recombinant Protein
• Search terms
  POU5F1 (Oct4) Recombinant Protein
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  POU5F1   Oct4   Protein  
• Gene symbol
  POU5F1
• Short name
  POU5F1 (Oct4) Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Primorigen advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens POU5F1 (Oct4) Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:9221
• Gene
  POU5F1
• Long gene name
  POU class 5 homeobox 1
• Synonyms gene
  • OTF3
• Synonyms gene name
  • POU domain class 5, transcription factor 1
• Synonyms
  • OCT3
  • Oct4
  • MGC22487
• GenBank acession
  • Z11898
• Locus
  6p21.33
• Discovery year
  1992-11-05
• Entrez gene record
  5460
• Pubmed identfication
  • 1408763
• RefSeq identity
  • NM_002701
• Classification
  • POU class homeoboxes and pseudogenes
• VEGA ID
  OTTHUMG00000031206

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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