Pou5f1 Antibody OCT4 (Zebrafish)
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Catalog number
F52574-0.08ML
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Price
Please ask
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Size
0.08 ml
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Category
Antibody
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Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Pou5f1 OCT4 (Zebrafish)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Zebrafish ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, ELISA
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Recommended dilutions
Western blot: 1:1000
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Notes
Titration of the Pou5f1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This Pou5f1 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
Q90270
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Purity
Antigen affinity
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Description
Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation.
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Immunogen
This Pou5f1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 128-161 amino acids from the N-terminal region of zebrafish Pou5f1.
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Storage
Aliquot the Pou5f1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
POU5F1P5, POU5F1P4, POU5F1
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Short name
Anti-Pou5f1 OCT4 (Zebrafish)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Species
Zebrafish, Zebrafishes
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Alternative name
Antibodies to Pou5f1 OCT4 (Zebrafish)
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Alternative technique
antibodies
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Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
POU class 5 homeobox 1
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Synonyms gene
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Synonyms gene name
- POU domain class 5, transcription factor 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-11-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- POU class homeoboxes and pseudogenes
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
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Tree numbers
- E01.370.225.500.620.670.325
- E01.370.225.750.600.670.325
- E05.200.500.620.670.325
- E05.200.750.600.670.325
- E05.393.661.475
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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