Recombinant Human Alkaline Phosphatase/ALPL (C-6His)
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Catalog numberC554-50
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Price:
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Size50 ug
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DescriptionRecombinant Human Alkaline Phosphatase is produced by our Mammalian expression system and the target gene encoding Leu18-Ser502 is expressed with a 6His tag at the C-terminus.
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Species reactivityHuman
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OriginHuman cells
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Peptide sequenceLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSVDHHHHHH
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Estimated molecular weight54,5 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionDry Ice/ice packs
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Package formSupplied as a 0.2 µm filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2, pH 7.5.
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Storage conditionsStore at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberP05186
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolALPL
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Short nameRecombinant Alkaline Phosphatase/ALPL (C-6His)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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LabelAlkaline Phosphatase
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SpeciesHuman, Humans
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Alternative nameHuman Alkaline Phosphatase/ALPL(C-6His)
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Alternative techniquerec
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Alternative to gene targetalkaline phosphatase, liver/bone/kidney, AP-TNAP and APTNAP and HOPS and TNAP and TNSALP, ALPL and IDBG-93194 and ENSG00000162551 and 249, metal ion binding, Extracellular, Alpl and IDBG-199139 and ENSMUSG00000028766 and 11647, ALPL and IDBG-645614 and ENSBTAG00000008951 and 280994
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Gene info
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Identity
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Gene
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Long gene namealkaline phosphatase, biomineralization associated
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Synonyms gene
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Synonyms gene name
- alkaline phosphatase, liver/bone/kidney
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Alkaline phosphatases
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data