Alkaline Phosphatase Antibody (liver)

• Catalog number
  F44129-0.08ML
• Price
  Please ask
• Size
  0.08 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Alkaline Phosphatase (liver)
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  FACS, IHC, WB, IF, ELISA
• Recommended dilutions
  Western blot: 1:1000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:10-1:50
• Notes
  Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Alkaline Phosphatase antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P05186
• Purity
  Purified
• Description
  There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.
• Immunogen
  A portion of amino acids 217-246 from human ALPL was used as the immunogen for this Alkaline Phosphatase antibody.
• Storage
  Aliquot the Alkaline Phosphatase antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Alkaline   Phosphatase   liver  
• Gene symbol
  ALPL
• Short name
  Anti-Alkaline Phosphatase (liver)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Label
  Alkaline Phosphatase
• Alternative name
  Antibodies to Alkaline Phosphatase (liver)
• Alternative technique
  antibodies
• Tissue
  liver

Gene info

• Identity
  HGNC:438
• Gene
  ALPL
• Long gene name
  alkaline phosphatase, biomineralization associated
• Synonyms gene
  • HOPS
• Synonyms gene name
  • alkaline phosphatase, liver/bone/kidney
• Synonyms
  • TNSALP
  • TNALP
  • TNAP
• Synonyms name
  • tissue non-specific alkaline phosphatase
• GenBank acession
  • BC021289
• Locus
  1p36.12
• Discovery year
  1986-01-01
• Entrez gene record
  249
• Pubmed identfication
  • 3532105
  • 3446011
• RefSeq identity
  • NM_000478
• Classification
  • Alkaline phosphatases
• VEGA ID
  OTTHUMG00000002949
• Locus Specific Databases
  • Tissue Nonspecific Alkaline Phosphatase Gene Mutation Database

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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