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Category
Proteins & Peptides
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Long description
Sphingolipids have emerged as central signaling molecules regulating a variety of fundamental cell responses such as cell death and differentiation, proliferation and inflammation. Ceramide has been a main focus of research since it possesses pro-apoptotic capacity in many cell types. Sphingosine-1-phosphate (S1P), which is generated from ceramide by the actions of ceramidase and sphingosine kinase can potently induce cell proliferation via binding to and activation of the S1P family of receptors. The balance between ceramide and sphingosine-1-phosphate determines whether cells undergo apoptosis or proliferate, two cell responses that are critically involved in tumor development. The amino acid sequence for sphingosine kionase 1a differs from sphingosine kinase 1 only in the absence of a valine at position 4 of sphingosine kinase 1a.
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Antibody come from
Synthetic peptide derived from the human SIAH-1 protein.
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Other description
Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
Sphingosine Kinase 1a (mouse recombinant antibody N-terminal His tag) - Active Enzyme
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Antibody is raised in
Sf9 cells
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Antibody s reacts with
Mouse
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Enzyme Kinetic studies, screening inhibitors, selectivity profiling
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Antibody s suited for
Antibody can be used for Western blotting (1-5ʵg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-80ºC
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Relevant references
1: Hu G., et al.; Mammalian homologs of seven in absentia regulate DCC via the ubiquitin-proteasome pathway.; Genes Dev. 11:2701-2714(1997)._x000B_2: Matsuzawa S., et al.; p53-inducible human homologue of Drosophila seven in absentia (Siah) inhibits cell growth: suppression by BAG-1.; EMBO J. 17:2736-2747(1998)._x000B_3: Hu G., Fearon E.R.; Siah-1 N-terminal RING domain is required for proteolysis function, and C-terminal sequences regulate oligomerization and binding to target proteins.; Mol. Cell. Biol. 19:724-732(1999)._x000B_4: Germani A., et al.; SIAH-1 interacts with alpha-tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis.; Oncogene 19:5997-6006(2000)._x000B_5: Tanikawa J., et al.; p53 suppresses the c-Myb-induced activation of heat shock transcription factor 3.; J. Biol. Chem. 275:15578-15585(2000)._x000B_6: Matsuzawa S., Reed J.C.; Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses.; Mol. Cell 7:915-926(2001)._x000B_7: Liu J., et al. Siah-1 mediates a novel beta-catenin degradation pathway linking p53 to the adenomatous polyposis coli protein.; Mol. Cell 7:927-936(2001)._x000B_8: Tiedt R., et al.; The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1.; EMBO J. 20:4143-4152(2001)._x000B_9: Boehm J., et al.; Regulation of BOB.1/OBF.1 stability by SIAH.; EMBO J. 20:4153-4162(2001)._x000B_10: Susini L., et al.; Siah-1 binds and regulates the function of Numb. Proc. Natl. Acad. Sci. U.S.A. 98:15067-15072(2001).
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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Conjugation
histidine
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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French translation
anticorps
