Sheep anti Bub1b-NT
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Catalog numberX1162P
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PricePlease ask
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Size250 μg
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CategoryPrimary Antibodies
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Long descriptionBub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.
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Antibody come fromRecombinant protein corresponding to amino acids 10-190 of the mouse Bub1b protein.
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Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
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ClonePolyclonal
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Antigen antibody binding interactionSheep anti Bub1b-NT Antibody
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Antibody is raised inSheep
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Antibody s reacts withMouse
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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ApplicationWestern Blot, Immunohistochemistry
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Antibody s suited forAntibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot analysis (1-10 µg/ml). Detects a 130 kDa band. Optimal concentration should be evaluated by serial dilutions.
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Storage-20ºC
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Relevant references1. Myrie, K.A., et al. Mutation and expression analysis of human Bub1 and Bub1b in aneuploid breast cancer cell lines. Cancer Lett. 2000, 152, 193-199._x000B__x000B_2. Davenport, J.W., et al. The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner. Genomics 1999, 55, 113-117.
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Gene target
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Gene symbolNTF4
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Short nameanti Bub1b-NT
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Techniqueanti, antibody to
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HostSheep
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Isotypenot specified
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Labelunlabeled
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SpeciesSheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative nameovine antibody to budding uninhibited by benzimidazoles 1 homolog b (yeast)-NT
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Alternative techniqueantibodies
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Alternative to gene targetbudding uninhibited by benzimidazoles 1 homolog beta (yeast), Bub1A and BUB1beta and BUBR1 and hBUBR1 and MAD3L and MVA1 and SSK1, BUB1B and IDBG-6103 and ENSG00000156970 and 701, transferase activity, nuclei, Bub1b and IDBG-198179 and ENSMUSG00000040084 and 12236, BT.25661 and IDBG-646208 and ENSBTAG00000007237 and 100851213,537027
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Gene info
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Identity
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Gene
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Long gene nameneurotrophin 4
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Synonyms gene
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Synonyms gene name
- neurotrophin 5 (neurotrophin 4/5)
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Synonyms
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Synonyms name
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Locus
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Discovery year1992-10-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Neurotrophins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data