Rat anti Rat anti-mouse IgG1k antibody conjugated to FITC

• Catalog number
  RG12
• Price
  Please ask
• Size
  100 Tests

• Category
  Second Step Reagents
• Long description
  Second Step reagents labeled with FITC or PE
• Antibody come from
  n/a
• Other description
  Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
• Clone
  not specified
• Antigen antibody binding interaction
  Rat anti Rat anti-mouse IgG1k antibody conjugated to FITC Antibody
• Antibody is raised in
  Rat
• Antibody s reacts with
  see techfile
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Flow Cytometry
• Antibody s suited for
  10 µl of purified reagent MAB / 100 µl of 10^6 PBMC (peripheral blood mononuclear cells) in PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and add either 10 µl of R M IgG1 PE or 10 µl of R M IgG1 FITC depending on the desired fluorescence and analyzed immediately. See instrument manufacturerÕs instructions for Immunofluorescence analysis with a flow cytometer or microscope.
• Storage
  4ºC
• Relevant references
  no information yet
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• French translation
  anticorps

• Gene target
  IgG1k   conjugated  
• Short name
  anti anti-mouse IgG1k antibody conjugated FITC
• Technique
  Antibody, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
• Host
  Rat
• Isotype
  not specified
• Label
  FITC
• Species
  Mouse, Mouses, Rats
• Alternative name
  Rat antibody to Rat Antibody tomouse IgG1k (antibody to-) coupled to fluorecein
• Alternative technique
  antibodies, murine, fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products