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Products_type
Antibody
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Products_short_name
[CD4]
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Products_name_syn
[CD4 Antibody, FITC; Rat Anti-Mouse CD4, FITC; gp55; HLA-2; L3 / T4; Ly-4; T cell antigen T4/LEU3; T4; sCD4; CD4mut; CD4 FITC]
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Other_names
[CD4 protein (CD4 receptor); CD4 protein; CD4 protein]
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Products_gene_name
[CD4]
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Other_gene_names
[CD4]
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Clonality
Monoclonal
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Clone
[mCD4]
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Purity
Ion exchange column.
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Concentration
1mg/ml in PBS (after reconstitution).
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Storage_stability
Lyophilized: store at 4 degree C. After reconstitution, if not intended for use within a month, aliquot and store at -20 degree C.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody. CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbol
CD4
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Short name
Anti Mouse CD4 FITC
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Technique
anti mouse, Mouse, anti, FITC, antibody to, anti mouse antibodies, Fluorescein, mouses
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Host
Rat
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Isotype
IgG
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Label
FITC
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Species
Mouse, Mouses, Rats
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Alternative name
Rat antibody to Mouse CD4 molecule fluorecein
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Alternative technique
murine, antibodies, fluorescine
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Alternative to gene target
CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
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