Rat anti E-selectin / CD62E
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Catalog numberMUB0504S
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PricePlease ask
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Size1 mL
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CategoryPrimary Antibodies
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Long descriptionLeukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFα and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFκB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
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Antibody come fromUZ4 is a Rat monoclonal IgM antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Lewis Rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
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Other descriptionEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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CloneUZ4
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Antigen antibody binding interactionRat anti E-selectin / CD62E Antibody
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Antibody is raised inRat
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Antibody s reacts withHuman,Mouse
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityUZ4 recognizes the minimal amino acid consensus sequence CXKKKL present in the lectin domain of both murine and Human E-selectin.
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Research interestCell adhesion,Immunology
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ApplicationFlow Cytometry,Immunofluorescence,Immunoprecipitation,Western blotting
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Antibody s suited forUZ4 is suitable for immunoblotting, ELISA, immunoprecipitation and flow cytometry (dilution 1:25). To analyze E-selectin protein in frozen sections we refer to the anti E-selectin monoclonal antibodies UZ5, UZ6, and UZ7. For a functional UZ4 antibody that inhibits leucocyte adhesion to murine and Human activated endothelial cells refer to the E-selectin antibody for in vivo use which does not contain azide.
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StorageStore at 4°C, or in small aliquots at -20°C.
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Relevant referencesHallmann, R., Zimmermann, U., Sorokin, L. M., Needham, L., and Von der Mark, K. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9. Austrup, F., Vestweber, D., Borges, E., Lohning, M., Brauer, R., Herz, U., Renz, H., Hallmann, R., Scheffold, A., Radbruch, A., and Hamann, A. (1997). P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues, Nature 385, 81-3. Borges, E., Tietz, W., Steegmaier, M., Moll, T., Hallmann, R., Hamann, A., and Vestweber, D. (1997). P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migRation into inflamed skin, J Exp Med 185, 573-8. Tietz, W., Allemand, Y., Borges, E., von Laer, D., Hallmann, R., Vestweber, D., and Hamann, A. (1998). CD4+ T cells migRate into inflamed skin only if they express ligands for E- and P-selectin, J Immunol 161, 963-70. Alferink, J., Tafuri, A., Vestweber, D., Hallmann, R., Hammerling, G. J., and Arnold, B. (1998). Control of neonatal tolerance to tissue antigens by peripheral T cell trafficking, Science 282, 1338-41. Wolf, D., Hallmann, R., Sass, G., Sixt, M., Kusters, S., Fregien, B., Trautwein, C., and Tiegs, G. (2001). TNF-alpha-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis, J Immunol 166, 1300-7. Hammel, M., Weitz-Schmidt, G., Krause, A., Moll, T., Vestweber, D., Zerwes, H. G., and Hallmann, R. (2001). Species-specific and conserved epitopes on Mouse and Human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74. Sunderkotter, C., Seeliger, S., Schonlau, F., Roth, J., Hallmann, R., Luger, T. A., Sorg, C., and Kolde, G. (2001). Different pathways leading to cutaneous leukocytoclastic vasculitis in mice, Exp Dermatol 10, 391-404. Kneilling, M., Reinhard, M., Hültner, L., Schönberger, T., Fuchs, K., Schaller, M., Bukala, D., Massberg, S., Sander, C.A., Braumüller, H., Eichner, M., Maier, K.L., Hallmann, R., Pichler, B.J., Haubner, R., Gawaz, M., Pfeffer, K., Biedermann, T., Röcken, M. (2009). Direct crosstalk between mast cell-TNF and TNFR1-expressing endothelia mediates local tissue inflammation. Blood, 114, 1696-1706.
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Protein numberP16581
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Gene symbolSELE
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Short nameanti E-selectin / CD62E
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Techniqueanti, antibody to
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HostRat
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IsotypeIgM
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Labelunlabeled
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SpeciesRat, Rats
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Alternative nameRat antibody to E-selectin / CD62E
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameselectin E
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Synonyms gene
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Synonyms gene name
- endothelial adhesion molecule 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
- CD molecules
- Sushi domain containing
- Selectins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data