Recombinant Rat E-Selectin / CD62e / SELE Protein (His tag) 200µg

• Catalog number
  PKSR030387-200µg
• Price
  Please ask
• Size
  200µg

• Synonym
  SELE
• Activity
  Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to rat E Selectin/Fc Chimera coated plates (2 μg/mL, 100 μL/well), approximately 30%-60% will adhere after 1 hour at 37℃. Optimal dilutions should be determined by each laboratory for each application.
• Sequence
  Met 1-Pro 494
• Fusion tag
  C-His
• Accession
  P98105-1
• ExpressedHost
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 97 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  53 kDa
• AP Mol Mass
  70-80 KDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. E-selectin regulates adhesive interactions between certain blood cells and endothelium. The soluble form of E selectin (sE-selectin) is a marker of endothelial activation, and has a potential role in the pathogenesis of cardiovascular disease as raised levels have been found in hypertension, diabetes and hyperlipidemia, although its association in established atherosclerosis disease and its value as a prognostic factor is more controversial. soluble E-selectin is inversely associated with the muscular component of the left ventricle, thereby suggesting that the lack of such a reparative factor may be associated with cardiac remodeling in end-stage renal disease (ESRD) patients. In addition, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.
• Technical Data
• Conjugation
  histidine
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  E-Selectin   CD62e   SELE   Protein   His   tag   200µg  
• Gene symbol
  SELE
• Short name
  Recombinant E-Selectin / CD62e / SELE Protein (His tag) 200µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  Rat
• Label
  His
• Species
  Rat, Rats
• Alternative name
  Rec. Rat E-Selectin / CD62e / selectin E Protein (histidine detection labelled) 200µg
• Alternative technique
  rec
• Alternative to gene target
  selectin E, CD62E and ELAM and ELAM1 and ESEL and LECAM2, SELE and IDBG-104701 and ENSG00000007908 and 6401, oli this GO saccharide binding, Extracellular, Sele and IDBG-202283 and ENSMUSG00000026582 and 20339, SELE and IDBG-631886 and ENSBTAG00000007307 and 281484

Gene info

• Identity
  HGNC:10718
• Gene
  SELE
• Long gene name
  selectin E
• Synonyms gene
  • ELAM1
  • ELAM
• Synonyms gene name
  • endothelial adhesion molecule 1
• Synonyms
  • ESEL
  • CD62E
• GenBank acession
  • M30640
• Locus
  1q24.2
• Discovery year
  1989-06-30
• Entrez gene record
  6401
• Pubmed identfication
  • 1375831
• RefSeq identity
  • NM_000450
• Classification
  • C-type lectin domain containing
  • CD molecules
  • Sushi domain containing
  • Selectins
• VEGA ID
  OTTHUMG00000034851

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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