-
Category
Primary Antibodies
-
Long description
Activation of Sphingosine kinase (Sphk) increases the bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Phosphorylation of sphingosine by sphingosine kinase (Sphk) is the rate-limiting step in the cellular synthesis of S1P. A principle activation event is the phosphorylation of serine 225 of sphiongosine kinase 1. Inhibition of Sphk1 phosphorylation and hence, Sphk activity is an important target for the development of drugs for cancer, inflammation, cardioprotection as well as a many other indications.
-
Antibody come from
Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant fragment of human FAK expressed in E. coli and mouse myeloma.
-
Other description
Provided as solution in phosphate buffered saline, pH 7.2, with 1%.BSA and 0.1% sodium azide. Antigen Immunoaffiinity Purification
-
Clone
Polyclonal
-
Antigen antibody binding interaction
Rabbit anti Sphingosine Kinase 1 antibody Mouse Antibody
-
Antibody is raised in
Rabbit
-
Antibody s reacts with
Q8CI15
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
Western Blot
-
Antibody s suited for
For Western blotting, the recommended concentration is 1 µg/mL. Optimal concentration should be evaluated by serial dilutions.
-
Storage
-80ºC
-
Relevant references
1. Cao, Y., et al. (2005) Growth factors stimulate kidney proximal tubule cell migration independent of augmented tyrosine phosphorylation of focal adhesion kinase. Biochem. Biophys. Res. Com. 328:560-566._x000B_2. Dahmani, S., et al. (2004) Lidocaine increases phosphorylation of focal adhesion kinase in rat hippocampal slices. Eur. J. Pharmacol. 489:55-58._x000B_3. Dunty, J., et al. (2004) FERM domain interaction promotes FAK signaling. Mol. Cell. Biol. 24(12):5353-5368._x000B_4. Ilic, D., et al. (2004) FAK promotes organization of fibronectin matrix and fibrillar adhesions. J. Cell Sci. 117:177-187._x000B_5. Lim, Y., et al. (2004) Phosphorylation of focal adhesion kinase at tyrosine 861 is crucial for Ras transformation of fibroblasts. J. Biol. Chem. 279(28):29060-29065._x000B_6. Lunn, J.A. and E. Rozengurt (2004) Hyperosmotic stress induces rapid focal adhesion kinase phosphorylation at tyrosines 397 and 577. J. Biol. Chem. 279(43):45266-45278._x000B_7. Romanova, L., et al. (2004) Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive. J. Cell Sci. 117:3759-3768._x000B_8. Hanks, S.K. and T.R. Polte (1997) Signaling through focal adhesion kinase. BioEssays 19(2):137-145._x000B_9. Ilic, D., C.H. Damsky (1997) Focal adhesion kinase: at the crossroads of signal transduction. J. Cell Sci. 110:401-407._x000B_10. Otey, C.A. (1996) pp125FAK in focal adhesion. Internat. Rev. Cytol. 167:161-183._x000B_11. Schlaepfer, D.D. and T. Hunter (1996) Signal transduction from the extracellular matrixÑa role for the focal adhesion protein-tyrosine kinase FAK. Cell Struct. Funct. 21(5):445-450._x000B_
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus, Oryctolagus cuniculus
-
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
French translation
anticorps
