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Category
Primary Antibodies
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Long description
Calcium/calmodulin kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers. The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr305 found in rat brain CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM kinase II with the PSD and reduce LTP and learning.
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Antibody come from
Synthetic peptide from rat voltage gated potassium channel, Kv2.2-subunit conjugated to keyhole limpet hemocyanin (KLH).
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Other description
Provided in HEPES (pH 7.5) solution containing 150 mM NaCl, 100 µg per ml BSA and 50% glycerol . Unpurified neat serum.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti CaM Kinase II (Thr305) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Bovine, Chicken, Human, Mouse, Rat, Xenopus
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Dot Blot
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Antibody s suited for
Antibody can be used for Westen blot and immunohistochemistry (1:1000 dilution). Optimal concentration should be evaluated by serial dilutions.
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Storage
-80ºC
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Relevant references
1. Blaine JT, Ribera AB (1998) Heteromultimeric potassium channels formed by members of the Kv2 subfamily. J Neurosci 18:9585-9593._x000B__x000B_2. Burger C, Ribera AB (1996) Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development. J Neurosci 16:1412-1421._x000B__x000B_3. Hwang PM, Fotuhi M, Bredt DS, Cunningham AM, Snyder SH (1993) Contrasting immunohistochemical localizations in rat brain of two novel K+ channels of the Shab subfamily. J Neurosci 13:1569-1576._x000B__x000B_4. Michaelevski I, Chikvashvili D, Tsuk S, Singer-Lahat D, Kang YH, Linial M, Gaisano HY, Fili O, Lotan I (2003) Direct interaction of target SNAREs with the Kv2.1 channel - Modal regulation of channel activation and inactivation gating. J Biol Chem 278:34320-34330._x000B__x000B_5. Pongs O (1999) Voltage-gated potassium channels: from hyperexcitability to excitement. FEBS Lett 452:31-35._x000B__x000B_6. Yan L, Figueroa DJ, Austin CP, Liu Y, Bugianesi RM, Slaughter RS, Kaczorowski GJ, Kohler MG (2004) Expression of voltage-gated potassium channels in human and rhesus pancreatic islets. Diabetes 53:597-607.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
