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Category
Primary Antibodies
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Long description
Calcium/calmodulin kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers. The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II. Phosphorylation of Thr286 produces an autonomously active form of CaM Kinase II.
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Antibody come from
Sythetic peptide corresponding to amino acid residues surrounding the phospho-Ser845 of rat GluR1.
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Other description
Provided in HEPES (pH 7.5) solution containing 150 mM NaCl, 100 µg per ml BSA and 50% glycerol . Affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti CaM Kinase II (Thr286) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human, Mouse, Rat, Xenopus
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Dot Blot
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Antibody s suited for
Antibody can be used for Westen blot (1:1000 dilution). Optimal concentration should be evaluated by serial dilutions.
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Storage
-80ºC
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Relevant references
1. Hollmann M, Heinemann S (1994) Cloned glutamate receptors. Annu Rev Neurosci 17:31-108._x000B__x000B_2. KeinŠnen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B, Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249:556-560._x000B__x000B_3. Mammen AL, Kameyama K, Roche KW, Huganir RL (1999) Phosphorylation of the a-amino-3-hydroxy-5- methylisoxazole-4-propionic acid receptor GluR1 subunit by calcium/calmodulin-dependent kinase II. J Biol Chem 272:32528-32533._x000B__x000B_4. McGlade-Mcculloh E, Yamamoto H, Tan S-E, Brickey DA, Soderling TR (1993) Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein kinase II. Nature (London) 362:640-642._x000B__x000B_5. Roche KW, O'Brien RJ, Mammen AL, Bernhardt J, Huganir RL (1996) Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit. Neuron 16:1179-1188._x000B__x000B_6. Soderling TR, Derkach VA (2000) Postsynaptic protein phosphorylation and LTP. Trends Neurosci 23:75-80.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
