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Category
Primary Antibodies
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Long description
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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Antibody come from
Ks8.7 is a Mouse monoclonal IgG1 antibody derived by fusion of murine myeloma cells with spleen cells from a Mouse immunized with cytoskeletal proteins from cultured HeLa cells.
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Other description
Each vial contains 1 ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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Clone
Ks8.7
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Antigen antibody binding interaction
Mouse anti Cytokeratin 8 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Hamster,Human,Mouse,Rat,Swine
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
Ks8.7 reacts with MCF-7, RT 112 and PLC cells as well as with adeno-carcinomas, undifferentiated carcinomas, cervix carcinomas and hepatocellular carcinomas.
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Research interest
Cytoskeleton
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Application
Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
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Antibody s suited for
Ks8.7 is suitable for immunoblotting, immunocytochemistry and for immunohistochemistry on frozen and paraffin embedded tissues with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution should be determined by titration.
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Storage
Store at 4oC, or in small aliquots at –20oC.
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Relevant references
Bartek, J., Vojtsek, B., Staskova, Z., Bartkova, J., Kerekes, Z., Rejthar, A., Kovraik, J. (1991). A series of 14 new monoclonal antibodies to Keratins: Characterizationi and value in diagnostic histophathology. J Pathol 164, 215-44. Moll, R., Franke, W.W., Schiller D.L., Geiger, B., Krepler, R. (1982). The Catalog of Human Cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24. Osborn, M., van Lessen, G., Weber, K., Klöppel, G., Altmannsberger, M. (1986). Methods in laboRatory investigation. Differential diagnosis of gastrointestinal carcinomas by using monoclonal antibodies specific for individual Keratin polypeptides. Lab. Investigation 55,4, 497-504. Gomi, H., Yokoyama, T., Fujimoto, K., Ikeda, T., Katoh, A., Itoh, T., Itohara, S. (1995). Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron, Vol. 14, 29-41. Stumptner, C., Fuchsbichler, A., Heid, H., Zatloukal, K., Denk, H. (2002). Mallory body - a disease-associated type of sequestosome. Hepatology 35, 1053-62.
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Protein number
P05787
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
