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Products_type
Antibody
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Products_short_name
[CYTOKERATIN 7/17]
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Other_names
[keratin, type I cytoskeletal 17; Keratin, type I cytoskeletal 17; keratin, type I cytoskeletal 17; 39.1; CK-17; cytokeratin-17; keratin 17 epitope S1; keratin 17 epitope S2; keratin 17 epitope S4; keratin 17, type I; 39.1; Cytokeratin-17; CK-17; Keratin-17; K17]
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Other_gene_names
[KRT17; KRT17; PC; K17; PC2; PCHC1; CK-17; K17]
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Clonality
Monoclonal
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Clone
[C-46]
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Reactivity
Bovine, Pig
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Specificity
This item specifically recognises a unique epitope shared by the intermediate filament keratins, cytokeratin 7 and cytokeratin 17, a 54kDa type II basic cytokeratin, and 46kDa type I acidic cytokeratin respectively. Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7. This item is reported as suitable for use in immunocytochemistry, see Bartek, J. et al. for details.
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Form
Purified Purified IgG - liquid
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Concentration
IgG concentration 1.0mg/ml
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Storage_stability
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
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Tested_application
Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
