Mouse anti CD56 antibody conjugated to PE
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Catalog number0564
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PricePlease ask
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Size100 Tests
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CategoryPrimary Antibodies
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Long descriptionCD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells.
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Antibody come fromCD56=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.
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Other descriptionProvided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein.
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CloneC5.9
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Antigen antibody binding interactionMouse anti CD56 antibody conjugated to PE Antibody
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Antibody is raised inMouse
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Antibody s reacts withHuman
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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Research interestCD Marker
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ApplicationFlow Cytometry
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Antibody s suited forPBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼ C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
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Storage4ºC
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Relevant references1. Generation of Monoclonal Antibodies to a Human Natural Killer Clone. Characterization of Two Natural Killer-Associated Antigens, NKH1 and NKH2, Expressed on Subset of Large Granular Lymphocytes. Hercend, T., Griffin, J.D., Benussan, A., Schmidt, R.E., Edson, M.A., Brennen, A., Murray, C., Daley, J.F., Schlossman, S.F., and Ritz, J.. J. Clin. Invest. 1985 75:932. _x000B__x000B_2. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) Antigen Expression on Human Peripheral Blood Nk Cells and Cytotoxic T Lymphocytes. Lanier, L.L., Le, A.M., Civin, C.I., Loken, M.R., and Phillips, J.H., J. Immunol. 1986 136,4480. _x000B__x000B_3. A subset of natural killer cells in peripheral blood displays a mature T cell phenotype. Schmidt RE, Murray C, Daley JF, Schlossman SF, and Ritz J, J Ex Med 1986 164: 351-356 _x000B__x000B_4. Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. Caligiuri M, Murray C, Buchwald D, Levine H, Cheney P, Peterson D, Komaroff AL, and Ritz J, J Immunol 1987 139:3306-3319 _x000B__x000B_5. Hercend T., and Schmidt, R.E., Immunol. Today 9,291,1988.
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesIf you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolNCAM1
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Short nameMouse anti CD56 antibody conjugated PE
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TechniqueAntibody, Mouse, anti, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, mouses
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Hostmouse
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IsotypeIgG2b
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LabelPE
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to CD56 (antibody to-) coupled to peroxidase
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Alternative techniqueantibodies, murine
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Gene info
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Identity
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Gene
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Long gene nameneural cell adhesion molecule 1
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- Fibronectin type III domain containing
- CD molecules
- Ig-like cell adhesion molecule family
- I-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data