TRAF6 Antibody
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Catalog numberR31345
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenTRAF6
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC-P, IHC-F, ICC
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Recommended dilutionsWestern blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
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NotesThe stated application concentrations are suggested starting amounts. Titration of the TRAF6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis TRAF6 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ9Y4K3
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PurityAntigen affinity
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DescriptionTNF receptor-associated factor 6, also called E3 ubiquitin-protein ligase TRAF6 or RNF85, is a TRAF human protein. The protein encoded by this gene is a member of the TNF receptor associated factor(TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This gene is mapped to 11p12. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase(IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein.
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ImmunogenAn amino acid sequence from the N-terminus of human TRAF6 (LRHLEDHQAHCEFALM) was used as the immunogen for this TRAF6 antibody.
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StorageAfter reconstitution, the TRAF6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolTRAF6
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Short nameAnti-TRAF6
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to TRAF6
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Alternative techniqueantibodies
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Alternative to gene targetTNF receptor-associated factor 6, E3 ubiquitin protein ligase, MGC:3310 and RNF85, TRAF6 and IDBG-40102 and ENSG00000175104 and 7189, protein N-terminus binding, nuclei, Traf6 and IDBG-193222 and ENSMUSG00000027164 and 22034, TRAF6 and IDBG-636763 and ENSBTAG00000036009 and 539124
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Gene info
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Identity
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Gene
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Long gene nameTNF receptor associated factor 6
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Synonyms gene name
- TNF receptor-associated factor 6, E3 ubiquitin protein ligase
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Synonyms
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Locus
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Discovery year1997-06-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ring finger proteins
- TNF receptor associated factors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data